A static biological leaching method was used to treat the 2,4-DNT-3-SA contaminated soil. The effects of oxygen conditions, soil to water ratio and temperature on the removal efficiency of 2,4-DNT-3-SA were evaluated. The result suggested that under the aerobic and 37ºC conditions, the removal efficiency of 2,4-DNT-3-SA reached more than 98% when the ratio of soil to water was 10:5. High-throughput sequencing results indicated that the dominant bacteria in the soil were Genus Pseudomonas, Sphingobium and Sporolactobacillus.
